COURSE OVERVIEW:

The meaning of this course lies in the mastery of the interaction between electromagnetic radiation and matter to quantify chemical substances. UV-Visible (UV-Vis) Spectrophotometry is one of the most ubiquitous and essential analytical techniques in the laboratory, yet its accurate application requires a deep understanding of the physics of light and the chemistry of the sample. This program provides the foundational scientific framework required to perform high-precision spectroscopic measurements for a wide range of industrial and research applications.

The scope of the training addresses the core principles of light absorption, including the Beer-Lambert Law and its physical and chemical limitations. It covers the essential hardware components of a spectrophotometer, from light sources and monochromators to detectors and cuvettes. Participants will explore the practical techniques of "Wavelength Selection," "Baseline Correction," and the development of "Standard Calibration Curves" for quantitative analysis.

The coverage includes a detailed investigation into the selection of appropriate solvents, the importance of "Photometric Accuracy," and the troubleshooting of common spectral interferences. The course emphasizes the importance of "Instrument Calibration" and "Performance Verification" to ensure that the data generated is accurate and legally defensible. By mastering these foundational spectroscopic protocols, participants will be able to perform reliable concentration measurements, identify unknown compounds, and maintain the performance of their spectroscopic instrumentation.

COURSE OBJECTIVES:

After completion of this course, the participants will be able to:

• Define the core principles of the Electromagnetic Spectrum and light absorption.
• Master the practical application of the Beer-Lambert Law.
• Identify the primary components of a modern UV-Vis Spectrophotometer.
• Select the appropriate "Wavelength" for maximum sensitivity and selectivity.
• Design a valid "Standard Calibration Curve" for quantitative analysis.
• Distinguish between Single-Beam and Double-Beam instrument designs.
• Choose and maintain the correct "Cuvette" type for UV and Visible ranges.
• Perform "Baseline Correction" and "Blanking" procedures correctly.
• Troubleshoot "Deviations" from the Beer-Lambert Law.
• Conduct "Performance Verification" (PV) for photometric accuracy.
• Calculate the "Concentration" of unknowns using regression data.
• Document spectrophotometric results for Quality Control reports.

TARGET AUDIENCE:

This course is intended for Junior Chemists, Laboratory Technicians, Quality Control Analysts, and University Science Students.

TRAINING COURSE METHODOLOGY:

A highly interactive combination of lectures, discussion sessions, and case studies will be employed to maximize the transfer of information, knowledge, and experience. The course will be intensive, practical, and highly interactive. The sessions will start by raising the most relevant questions and motivating everybody to find the right answers. The attendants will also be encouraged to raise more of their questions and to share in developing the right answers using their analysis and experience. There will also be some indoor experiential activities to enhance the learning experience. Course material will be provided in PowerPoint, with necessary animations, learning videos, and general discussions.

The course participants shall be evaluated before, during, and at the end of the course.

COURSE CERTIFICATE:

National Consultant Centre for Training LLC (NCC) will issue an Attendance Certificate to all participants completing a minimum of 80% of the total attendance time requirement.