COURSE OVERVIEW:

The meaning of this course lies in the technical mastery of High-Performance Liquid Chromatography (HPLC) as a cornerstone of modern analytical chemistry. While many operators can perform routine injections, true proficiency requires a deep understanding of the delicate balance between the mobile phase, the stationary phase, and the instrument hardware. This program provides the competencies needed to deliver reproducible and accurate results while ensuring the long-term reliability of the chromatographic system.

The scope of the training encompasses the entire HPLC analytical cycle, from mobile phase preparation and column equilibration to detector optimization and data integration. It addresses the mechanical intricacies of high-pressure pumps, autosamplers, and column compartments, emphasizing the importance of preventative maintenance. Participants will explore the principles of Reversed-Phase chromatography, the management of gradient elution profiles, and the calibration protocols required for high-stakes quantitative analysis.

The coverage includes a rigorous investigation into system troubleshooting, focusing on the identification and resolution of common issues such as pressure fluctuations, baseline noise, and peak tailing. The course emphasizes the interpretation of "system suitability" data and the execution of performance qualification (PQ) tests. By mastering these HPLC protocols, analysts will be able to minimize instrument downtime, optimize method performance, and ensure that every chromatographic run meets the highest standards of data integrity.

COURSE OBJECTIVES:

After completion of this course, the participants will be able to:

• Explain the physical and chemical principles of HPLC separations.
• Prepare mobile phases with precise pH and buffer concentrations.
• Optimize pump parameters for stable and pulse-free flow rates.
• Configure autosamplers for high-precision and low-carryover injections.
• Select the appropriate HPLC column based on analyte characteristics.
• Interpret UV-Vis and Photodiode Array (PDA) detector signals.
• Execute internal and external calibration for quantitative assays.
• Troubleshoot high-pressure leaks and solvent delivery failures.
• Resolve common chromatographic artifacts like peak tailing and ghosting.
• Perform routine preventative maintenance on HPLC modules.
• Conduct System Suitability Tests (SST) to ensure data validity.
• Document HPLC results according to GLP and ISO standards.

TARGET AUDIENCE:

This course is intended for Analytical Chemists, Quality Control Technicians, Pharmaceutical Analysts, and Laboratory Supervisors using HPLC technology.

TRAINING COURSE METHODOLOGY:

A highly interactive combination of lectures, discussion sessions, and case studies will be employed to maximize the transfer of information, knowledge, and experience. The course will be intensive, practical, and highly interactive. The sessions will start by raising the most relevant questions and motivating everybody to find the right answers. The attendants will also be encouraged to raise more of their questions and to share in developing the right answers using their analysis and experience. There will also be some indoor experiential activities to enhance the learning experience. Course material will be provided in PowerPoint, with necessary animations, learning videos, and general discussions.

The course participants shall be evaluated before, during, and at the end of the course.

COURSE CERTIFICATE:

National Consultant Centre for Training LLC (NCC) will issue an Attendance Certificate to all participants completing a minimum of 80% of the total attendance time requirement.