COURSE OVERVIEW:

The meaning of this course lies in the intellectual ability to design robust Gas Chromatography (GC) methods from a blank page. Method development is a scientific art that requires balancing resolution, speed, and sensitivity. This program provides the systematic framework necessary to move beyond "standard methods" and create customized analytical protocols that are optimized for specific industrial challenges and difficult sample matrices.

The scope of the training focuses on the logical selection of the stationary phase, the optimization of the oven temperature program, and the configuration of the detector for maximum signal-to-noise. It addresses the critical steps of sample preparation, inlet optimization, and the establishment of statistically sound calibration models. Participants will explore the use of "Retention Indices" and "Spectral Libraries" for the confident identification of unknown components in complex mixtures.

The coverage includes a deep investigation into the troubleshooting of chromatographic artifacts, such as peak tailing, co-elution, and ghost peaks. The course emphasizes the importance of "Method Validation" (Accuracy, Precision, Linearity) and the calculation of the "Limit of Detection." By mastering these method development strategies, participants will be able to deliver high-quality analytical data that is both technically defensible and economically viable for high-throughput laboratory environments.

COURSE OBJECTIVES:

After completion of this course, the participants will be able to:

• Select the optimal GC column based on analyte polarity and boiling point.
• Design efficient "Oven Temperature Programs" to resolve complex peaks.
• Optimize the "Split Ratio" and "Injection Volume" for trace analysis.
• Implement effective sample preparation (Headspace, SPME, Liquid-Liquid).
• Create and validate "Linear" and "Non-Linear" calibration models.
• Use "Internal Standards" to improve quantitative precision.
• Identify and eliminate "Ghost Peaks" and systemic contamination.
• Troubleshoot "Retention Time Shifts" and "Loss of Resolution."
• Calculate the "Method Detection Limit" (MDL) statistically.
• Utilize "Kovats Retention Indices" for component identification.
• Validate GC methods according to ICH and ISO requirements.
• Document a "Method Development Report" for regulatory compliance.

TARGET AUDIENCE:

This course is intended for Method Development Chemists, Senior Analysts, Quality Control Supervisors, and Research Scientists using GC technology.

TRAINING COURSE METHODOLOGY:

A highly interactive combination of lectures, discussion sessions, and case studies will be employed to maximize the transfer of information, knowledge, and experience. The course will be intensive, practical, and highly interactive. The sessions will start by raising the most relevant questions and motivating everybody to find the right answers. The attendants will also be encouraged to raise more of their questions and to share in developing the right answers using their analysis and experience. There will also be some indoor experiential activities to enhance the learning experience. Course material will be provided in PowerPoint, with necessary animations, learning videos, and general discussions.

The course participants shall be evaluated before, during, and at the end of the course.

COURSE CERTIFICATE:

National Consultant Centre for Training LLC (NCC) will issue an Attendance Certificate to all participants completing a minimum of 80% of the total attendance time requirement.