COURSE OVERVIEW:

The meaning of this course lies in the synergy between separation science and molecular identification, providing the ultimate toolkit for complex chemical analysis. Gas Chromatography-Mass Spectrometry (GC/MS) is the definitive technique for identifying unknown substances and quantifying trace levels in difficult matrices. This course moves beyond the "black box" operation to provide a deep understanding of how ions are formed, separated, and detected in the gas phase.

The scope of this training involves the detailed study of various ionization techniques and mass analyzer architectures, including single quadrupole, triple quadrupole (QQQ), and Time-of-Flight (TOF) systems. It addresses the critical interface between the GC and the MS, focusing on vacuum technology and high-temperature transfer lines. By mastering spectral interpretation and library searching, participants will be able to confirm molecular structures with a high degree of confidence.

The coverage includes advanced troubleshooting of common GC/MS issues, such as air leaks, source contamination, and electronic noise. Participants will learn how to optimize SIM (Selected Ion Monitoring) and MRM (Multiple Reaction Monitoring) methods for maximum sensitivity. This course provides the technical foundation necessary to solve complex analytical problems in forensic science, environmental monitoring, and food safety using the most powerful tools in modern chemistry.

COURSE OBJECTIVES:

After completion of this course, the participants will be able to:

• Explain the physical principles of various mass spectrometry ion sources.
• Optimize GC parameters to ensure compatibility with MS vacuum systems.
• Interpret Electron Impact (EI) fragmentation patterns for unknown identification.
• Utilize Chemical Ionization (CI) for molecular weight confirmation.
• Configure and tune the mass analyzer for maximum resolution and sensitivity.
• Master the use of mass spectral libraries and search algorithms.
• Develop robust quantitative methods using internal standards and isotopes.
• Troubleshoot vacuum system failures and diffusion/turbomolecular pumps.
• Perform routine maintenance on the ion source, filaments, and multipliers.
• Identify and eliminate sources of "background noise" and "leak" signals.
• Execute advanced data processing using deconvolution and filtering.
• Validate GC/MS methods for regulatory and forensic applications.

TARGET AUDIENCE:

This course is designed for Analytical Chemists, Forensic Scientists, Research Researchers, and Senior Technicians who use GC/MS for qualitative and quantitative chemical analysis.

TRAINING COURSE METHODOLOGY:

A highly interactive combination of lectures, discussion sessions, and case studies will be employed to maximize the transfer of information, knowledge, and experience. The course will be intensive, practical, and highly interactive. The sessions will start by raising the most relevant questions and motivating everybody to find the right answers. The attendants will also be encouraged to raise more of their questions and to share in developing the right answers using their analysis and experience. There will also be some indoor experiential activities to enhance the learning experience. Course material will be provided in PowerPoint, with necessary animations, learning videos, and general discussions.

The course participants shall be evaluated before, during, and at the end of the course.

COURSE CERTIFICATE:

National Consultant Centre for Training LLC (NCC) will issue an Attendance Certificate to all participants completing a minimum of 80% of the total attendance time requirement.