COURSE OVERVIEW:

The meaning of this course lies in the fundamental mastery of separation science as the primary tool for isolating chemical components from complex mixtures. Chromatography is the most widely used analytical technique in the world, yet its effectiveness depends entirely on the operator's understanding of the interactions between the mobile and stationary phases. This program provides the foundational theory and practical skills required to perform reliable separations in a modern laboratory.

The scope of the training introduces the core concepts of both Liquid Chromatography and Gas Chromatography, focusing on the mechanics of molecular movement through a column. It addresses the physical and chemical properties that drive separation, such as polarity, size, and volatility. Participants will explore the various hardware components common to all chromatographic systems, including pumps, injectors, columns, and detectors, providing a holistic view of the system's architecture.

The coverage includes essential topics such as peak integration, retention time stability, and the impact of flow rate and temperature on separation efficiency. Attendees will learn to interpret chromatograms, identify common separation artifacts, and perform basic system maintenance. By establishing a solid technical baseline, the course empowers laboratory staff to move beyond automated operation toward a more critical and informed approach to chromatographic analysis.

COURSE OBJECTIVES:

After completion of this course, the participants will be able to:

• Define the core principles of the chromatographic separation process.
• Distinguish between the mechanisms of adsorption, partition, and ion exchange.
• Identify and describe the function of all major HPLC and GC components.
• Select the appropriate mobile phase and column for basic separations.
• Interpret the physical meaning of retention time and peak area.
• Calculate critical parameters including resolution, capacity factor, and efficiency.
• Optimize flow rates and temperature to improve peak resolution.
• Perform basic peak integration and baseline correction in data software.
• Execute routine maintenance tasks such as seal replacement and liner changes.
• Troubleshoot common issues like peak tailing, fronting, and ghost peaks.
• Prepare samples and standards to ensure compatibility with the system.
• Comply with safety protocols for handling flammable solvents and high-pressure gases.

TARGET AUDIENCE:

This course is intended for Entry-level Chemists, Laboratory Technicians, Quality Control Analysts, and Chemical Engineers who are new to the field of chromatographic analysis.

TRAINING COURSE METHODOLOGY:

A highly interactive combination of lectures, discussion sessions, and case studies will be employed to maximize the transfer of information, knowledge, and experience. The course will be intensive, practical, and highly interactive. The sessions will start by raising the most relevant questions and motivating everybody to find the right answers. The attendants will also be encouraged to raise more of their questions and to share in developing the right answers using their analysis and experience. There will also be some indoor experiential activities to enhance the learning experience. Course material will be provided in PowerPoint, with necessary animations, learning videos, and general discussions.

The course participants shall be evaluated before, during, and at the end of the course.

COURSE CERTIFICATE:

National Consultant Centre for Training LLC (NCC) will issue an Attendance Certificate to all participants completing a minimum of 80% of the total attendance time requirement.